The Ontogeny of Blood Oxygen Transport and the Hypoxia Response in Early Life Stages of the Rainbow Trout, Oncorhynchus mykiss

dc.contributor.advisorWright, Pat
dc.contributor.authorBianchini, Kristin
dc.date.accessioned2012-11-13T14:45:20Z
dc.date.available2012-11-13T14:45:20Z
dc.date.copyright2012-10
dc.date.created2012-10-30
dc.date.issued2012-11-13
dc.degree.departmentDepartment of Integrative Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeIntegrative Biologyen_US
dc.description.abstractIn early rainbow trout development, a switch from high-affinity embryonic hemoglobin to lower-affinity adult hemoglobin occurs along with a turnover of round, embryonic erythrocytes to oval, adult erythrocytes. The objective of my thesis was to determine how the ontogeny of blood oxygen transport was affected by chronic hypoxia (30% of saturation) in rainbow trout. Hemoglobin-oxygen affinity, cooperativity, and the Bohr and Root effects were unaffected by hypoxia treatments, whereas hemoglobin content, erythrocyte counts, and hematocrit were significantly reduced. In hypoxia, larvae had higher concentrations of embryonic hemoglobin mRNA and embryonic erythrocytes than stage-matched normoxia-reared larvae. Overall, these results indicate that chronic hypoxia suppresses erythrocyte development prior to complete yolk absorption. Ultimately, this suggests that in early ontogeny rainbow trout conform to low oxygen conditions, rather than mounting the hypoxia response observed in oxygen-regulating adult trout.en_US
dc.identifier.urihttp://hdl.handle.net/10214/4323
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rightsAttribution-NonCommercial-NoDerivs 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ca/*
dc.subjectHypoxiaen_US
dc.subjectDevelopmental biologyen_US
dc.subjectHemoglobinen_US
dc.subjectBlooden_US
dc.subjectRainbow trouten_US
dc.titleThe Ontogeny of Blood Oxygen Transport and the Hypoxia Response in Early Life Stages of the Rainbow Trout, Oncorhynchus mykissen_US
dc.typeThesisen_US

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