From One to One Hundred: Scaling an Environmental DNA Sampling Program for Monitoring of the Invasive Sea Lamprey (Petromyzon marinus)

dc.contributor.advisorHanner, Robert
dc.contributor.advisorDocker, Margaret
dc.contributor.authorBrown, Cameron
dc.date.accessioned2023-09-14T14:03:10Z
dc.date.available2023-09-14T14:03:10Z
dc.date.copyright2023-09
dc.date.created2023-08-31
dc.degree.departmentDepartment of Integrative Biology
dc.degree.grantorUniversity of Guelphen
dc.degree.nameMaster of Science
dc.degree.programmeIntegrative Biology
dc.description.abstractIt is unclear if environmental DNA (eDNA) methods can be used for routine monitoring of the invasive Sea Lamprey (Petromyzon marinus). Previous research demonstrated that eDNA can detect invasive Sea Lamprey in both lab and field settings. However, the eDNA methods had not been optimized and validated for routine application. Three experiments were conducted to compare methods of eDNA field sample collection and create an optimized protocol. Then, eDNA sampling was benchmarked against standardized electrofishing at 54 sampling stations on 12 Great Lakes tributaries. eDNA detections (presence/absence) matched electrofishing detections at 79.6% of sampling stations. eDNA methods detected the novel presence of Sea Lamprey at 18.5% of sampling stations, and eDNA failed to detect electrofishing verified Sea Lamprey presence at 1.9% of sampling stations, demonstrating that Sea Lamprey detections from eDNA methods are highly congruent with detections from standard electrofishing protocols.
dc.description.sponsorshipGreat Lakes Fishery Commission
dc.identifier.urihttps://hdl.handle.net/10214/27924
dc.language.isoenen
dc.publisherUniversity of Guelphen
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.en
dc.subjectEnvironmental DNA
dc.subjectSea Lamprey
dc.subjectGreat Lakes
dc.titleFrom One to One Hundred: Scaling an Environmental DNA Sampling Program for Monitoring of the Invasive Sea Lamprey (Petromyzon marinus)
dc.typeThesisen

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Brown_Cameron_202309_MSc.pdf
Size:
3.78 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
353 B
Format:
Item-specific license agreed upon to submission
Description: