Identification of epigenetic, metabolic, and morphological variation between standardized somatic cell donors

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Chan, Biankha
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University of Guelph

Variation in the reprogramming capacity of donor cell lines significantly lowers the potential of SCNT and iPSC generation. This study revealed that normalized bovine donors and their subsequent standardized cell lines do not exhibit remarkable differences in the epigenetic, metabolic, and morphological parameters chosen to evaluate. No differences were found in the level of DNA (de)methylation and several repressive (H3K9me3 and H3K27me3)/permissive (H3K9ac and H3K27ac) histone marks, nor did cell lines differ in the level of metabolic metabolites, acetyl-CoA and αKG. However, Bull 13 was found to have a significantly lower number of SKPs that were >200 μm in size and had a higher number of SKPs that were <100 μm. Furthermore, Bull 14 and 15 had the highest level of H3K4me3, a permissive chromatin mark, while Bull 1 and 4 had the lowest. While differences were observed, their correlation with reprogramming ability is yet to be evaluated.

Somatic cell nuclear transfer, Induced pluripotent stem cells, Epigenetics, Metabolism, Nuclear reprogramming