Soy derived liposomes improve the long term viability of stored boar sperm

Tisch, Adrianne Mae
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University of Guelph

This research hypothesized that soy-based lipids in extenders would maintain viability, motility, and acrosomal status of boar sperm for seven days, while eliminating bio-security concerns. Individual ejaculates (n=5) were split and treated with one of 6 treatments in BTS extender or BTS alone (control). Treatments were soy-based liposomes (0.3, 0.5, 1.5 or 2 mg/ml), a synthetic liposome (0.3 mg/ml), a combination of the modified synthetic plus soy liposome (1:1, wt:wt, 0.3 mg/ml). Liquid semen was stored at 18°C for seven days, checking viability, motility and acrosomal status on days 0, 3 and 7. By Day 7 of storage, 30% more sperm were alive in the treatment groups than the control, although motility did not differ. Acrosomal status also differed among treatments, with sperm in soy 0.3mg/ml on day 0 and soy 0.5mg/ml on day 3 at 6hr decreasing in intact cells when compared to the control. Soy 1.5mg/ml was found to be the best concentration for sperm viability.

soy-based lipids, viability, motility, acrosomal status, boar sperm, bio-security concerns