As an economically significant industry in Ontario, dairy farming is increasingly challenged for ecologically-damaging production practices adversely impacting biodiversity and ecosystem services. Emerging campaigns in Ontario encourage farmers to not only mitigate such adverse effects but work to have production benefit biodiversity resources and vice versa. In this context, however, agricultural biodiversity conservation is dependent on farmers' voluntary actions and available supports. This case study research characterizes agri-environmental practices and program delivery in southwestern Ontario's dairy industry to support farmers' efforts effectively. Results reveal that farmers' motivations for biodiversity conservation are complex, having implications for program design and delivery. Results also show that conservation authorities are prominent in delivering programs to farmers. However, the capacity of conservation authorities to continue doing so may be limited by legislative changes to their mandates and partnering municipalities' capacity. This research concludes with recommendations to improve programs and outlines an inventory of analyzed programs.