Consumption of an EPA and DHA rich diet rescues changes in White Adipose Tissue Lipid Metabolism and Oxylipin Production induced with a Fads2 Deficiency

dc.contributor.advisorMutch, David
dc.contributor.authorPayne, George William
dc.date.accessioned2015-10-26T13:29:50Z
dc.date.available2015-10-26T13:29:50Z
dc.date.copyright2015-09
dc.date.created2015-10-19
dc.date.issued2015-10-26
dc.degree.departmentDepartment of Human Health and Nutritional Sciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeHuman Health and Nutritional Sciencesen_US
dc.description.abstractThe primary objective of this thesis was to examine the role of fatty acid desaturase 2 (Fads2) in white adipose tissue (WAT). A secondary objective was to assess whether the consumption of a diet supplemented with menhaden oil (MD), which is rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), could rescue changes in WAT associated with a Fads2 deficiency. Fads2-/- mice were found to have a lower bodyweight than mice fed the MD. Additionally, Fads2-/- mice exhibited changes in the expression of genes related to polyunsaturated fatty acid (PUFA) metabolism, which were abrogated with MD feeding. EPA and DHA were depleted in WAT of Fads2-/- mice, as were their derived oxylipins; however, feeding Fads2-/- mice MD restored both omega-3 PUFA and oxylipin profiles. Overall, this thesis demonstrates that the changes in WAT associated with a Fads2 deficiency can be rescued with a nutritional intervention containing EPA and DHA.en_US
dc.description.sponsorshipOntario Graduate Scholarship
dc.identifier.urihttp://hdl.handle.net/10214/9305
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rightsAttribution-NonCommercial-NoDerivs 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ca/*
dc.subjectWhite Adipose Tissueen_US
dc.subjectOmega-3 Polyunsaturated Fatty Acidsen_US
dc.subjectEicosapentaenoic Aciden_US
dc.subjectDocosahexaenoic Aciden_US
dc.subjectFatty Acid Desaturase 2en_US
dc.subjectKnockout Miceen_US
dc.subjectAlpha-linolenic Aciden_US
dc.subjectLipid Metabolismen_US
dc.subjectOxylipinsen_US
dc.titleConsumption of an EPA and DHA rich diet rescues changes in White Adipose Tissue Lipid Metabolism and Oxylipin Production induced with a Fads2 Deficiencyen_US
dc.typeThesisen_US

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