Consumption of an EPA and DHA rich diet rescues changes in White Adipose Tissue Lipid Metabolism and Oxylipin Production induced with a Fads2 Deficiency
The primary objective of this thesis was to examine the role of fatty acid desaturase 2 (Fads2) in white adipose tissue (WAT). A secondary objective was to assess whether the consumption of a diet supplemented with menhaden oil (MD), which is rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), could rescue changes in WAT associated with a Fads2 deficiency. Fads2-/- mice were found to have a lower bodyweight than mice fed the MD. Additionally, Fads2-/- mice exhibited changes in the expression of genes related to polyunsaturated fatty acid (PUFA) metabolism, which were abrogated with MD feeding. EPA and DHA were depleted in WAT of Fads2-/- mice, as were their derived oxylipins; however, feeding Fads2-/- mice MD restored both omega-3 PUFA and oxylipin profiles. Overall, this thesis demonstrates that the changes in WAT associated with a Fads2 deficiency can be rescued with a nutritional intervention containing EPA and DHA.