Investigation of Starch Synthesis in a TILLING Mutant of Maize

dc.contributor.advisorEmes, Michael
dc.contributor.authorMassey, Sarah
dc.date.accessioned2014-05-06T16:20:31Z
dc.date.available2014-05-06T16:20:31Z
dc.date.copyright2014-04
dc.date.created2014-05-02
dc.date.issued2014-05-06
dc.degree.departmentDepartment of Molecular and Cellular Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeMolecular and Cellular Biologyen_US
dc.description.abstractStarch is an important polymer made up of amylose and amylopectin. ADP-glucose pyrophosphorylase (AGPase), starch synthases (SS), starch branching enzymes (SBE), and starch debranching enzymes (SDE) are involved in starch synthesis. SSI, SSIIa and SBEIIb associate in phosphorylation-dependent multi-subunit complexes in maize. This investigation aimed to characterize new mutations in the SBEIIb gene using a TILLING technique. A single nucleotide substitution (G>A) in the SBEIIb gene was generated, translating to a cysteine to tyrosine amino acid change (C663Y) in a putative protein-protein interaction domain. C663Y SBEIIb appears to have reduced activity compared to the reference genotype, and the SBEIIb-SSIIa interaction is weakened. Starch granules from the C663Y mutant appear to possess SBEIIb as well as another branching isoform, SBEI, which is not normally found in the starch granule. The work presented here suggests altered protein-protein interactions in the C663Y mutant resulting in a change in starch structure.en_US
dc.description.sponsorshipOntario Ministry of Agriculture, Food and Rural Affairs
dc.identifier.urihttp://hdl.handle.net/10214/8067
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectStarchen_US
dc.subjectStarch Branching Enzymeen_US
dc.subjectProtein-Protein Interactionsen_US
dc.subjectResistant Starchen_US
dc.subjectProtein Complexesen_US
dc.subjectMaizeen_US
dc.titleInvestigation of Starch Synthesis in a TILLING Mutant of Maizeen_US
dc.typeThesisen_US

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