Investigation of host receptors involved in Bordetella bronchiseptica infection

dc.contributor.advisorPreston, A.
dc.contributor.authorCock, Jessica Lyn
dc.date.accessioned2020-08-24T15:51:23Z
dc.date.available2020-08-24T15:51:23Z
dc.date.copyright2007
dc.degree.departmentDepartment of Molecular and Cellular Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractBordetella bronchiseptica' infection in canines can result in tracheobronchitis (commonly known as kennel cough). An essential early step in 'B. bronchiseptica' infection is attachment of the bacterium to the cilia present on respiratory epithelial cells. I hypothesize that the receptors on host tissue utilized by 'B. bronchiseptica' are glycosphingolipids. Bacterial overlay experiments were conducted to identify species of glycolipid that bind 'B. bronchiseptica'. Solid-phase adherence assays were also performed using various pure glycolipids. The results suggest that lactosylceramide is a ligand for 'B. bronchiseptica' binding and that this binding requires the presence of both adhesins filamentous hemagglutinin (FHA) and fimbriae (FIM). GC-MS conducted on lipid preparations of canine cilia showed glucose, galactose, and 'N'-acetylglucosamine as the major sugar species present. Inhibition studies have shown that the lectin, wheat germ agglutinin, blocks binding of 'B. bronchiseptica ' to canine ciliated epithelial cells suggesting that 'N'-acetylglucosamine or sialic acid may be a component of the receptor.en_US
dc.identifier.urihttps://hdl.handle.net/10214/20389
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectBordetella bronchisepticaen_US
dc.subjectinfectionen_US
dc.subjectcaninesen_US
dc.subjecttracheobronchitisen_US
dc.subjectkennel coughen_US
dc.subjectbacteriumen_US
dc.subjectrespiratory epithelial cellsen_US
dc.titleInvestigation of host receptors involved in Bordetella bronchiseptica infectionen_US
dc.typeThesisen_US

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