Investigation of regulatory elements directing Dlx gene expression in the avian forebrain

dc.contributor.advisorBendall, A.
dc.contributor.authorWilliams, Lesedi
dc.date.accessioned2021-04-20T15:28:58Z
dc.date.available2021-04-20T15:28:58Z
dc.date.copyright2010
dc.degree.departmentDepartment of Molecular and Cellular Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractThere are six known mammalian 'Distal-less' type homeobox ('Dlx') genes, arranged in three convergently transcribed pairs. Highly conserved 'cis'-acting enhancer sequences have been identified in the 'Dlx1/2' and 'Dlx5/6' intergenic regions in zebrafish and mouse. 'Dlx3' and ' Dlx4' were originally thought not to be expressed in the forebrain of any vertebrate embryo; however expression of 'Dlx3' in the ventral forebrain of chick embryos has since been documented. Based on current models of 'Dlx' gene evolution it is unclear whether forebrain enhancers have been retained in the 'Dlx3/4' locus throughout the course of avian evolution or whether they were acquired after the divergence of birds from other vertebrate lineages. It is difficult to evaluate forebrain enhancer conservation in the avian lineage with the current available genome sequence. This thesis describes the isolation of a genomic clone containing a partial 'Dlx3' gene and sequences downstream, and the development of assays to test enhancer activity in chicken and zebrafish embryos.en_US
dc.identifier.urihttps://hdl.handle.net/10214/25200
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectmammalianen_US
dc.subjectDistal-less type homeobox genesen_US
dc.subjectDlx3 geneen_US
dc.subjectregulationen_US
dc.subjectexpressionen_US
dc.titleInvestigation of regulatory elements directing Dlx gene expression in the avian forebrainen_US
dc.typeThesisen_US

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