EPA and DHA Modulate Macrophage-Derived Inflammation and Subsequent Skeletal Muscle Inflammation

dc.contributor.advisorRobinson, Lindsay
dc.contributor.authorSepa-Kishi, Diane
dc.date.accessioned2013-09-07T20:28:51Z
dc.date.available2013-09-07T20:28:51Z
dc.date.copyright2013-08
dc.date.created2013-08-29
dc.date.issued2013-09-07
dc.degree.departmentDepartment of Human Health and Nutritional Sciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeHuman Health and Nutritional Sciencesen_US
dc.description.abstractMacrophage-derived inflammation contributes to chronic inflammation in adipose tissue in obesity and is also linked to the development of skeletal muscle (SM) insulin resistance. The long-chain n-3 PUFA have been shown to modulate cytokine secretion from macrophages, though subsequent effects on SM inflammation and function are unknown. A model of macrophage conditioned media (MCM) was used to examine effects of n-3 PUFA on macrophage inflammation and consequent effects on SM cells. Treatment of RAW 264.7 macrophages with long-chain n-3 PUFA decreased LPS-induced MCP-1 and IL-6 gene expression and MCP-1 secreted protein. In turn, MCM from n-3 PUFA-treated macrophages decreased TNF-α and IL-6 gene expression in LPS-stimulated L6 SM cells, but did not affect insulin-stimulated pAkt content. Long-chain n-3 PUFA did not affect gene expression of inflammatory signaling intermediates NF-κB and TLR4. Overall this thesis suggests that long-chain n-3 PUFA are important nutritional strategies for reducing macrophage-derived inflammation, with ensuing benefits in SM inflammation.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada - CGS
dc.description.sponsorshipOntario Graduate Scholarship
dc.identifier.urihttp://hdl.handle.net/10214/7484
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectmacrophage-derived inflammationen_US
dc.subjectadipose tissueen_US
dc.subjectskeletal muscleen_US
dc.subjectn-3 polyunsaturated fatty acidsen_US
dc.subjectmacrophage conditioned mediaen_US
dc.subjectLPS-induced inflammationen_US
dc.titleEPA and DHA Modulate Macrophage-Derived Inflammation and Subsequent Skeletal Muscle Inflammationen_US
dc.typeThesisen_US

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