Expression of foreign gene sequences mediated by chicken lysozyme gene regulatory sequences

dc.contributor.advisorGibbins, Ann
dc.contributor.authorLampard, Gregory Raymond of Animal and Poultry Scienceen_US of Guelphen_US of Scienceen_US
dc.description.abstractUse of the chicken lysozyme gene regulatory domain may facilitate the production of a transgenic chicken bioreactor capable of producing valuable proteins deposited in egg white. Research reported here illustrates that sequences encoding green fluorescent protein (GFP) can be fused to the 3' terminus of the lysozyme signal peptide encoding sequence and be expressed correctly under the control of the cytomegalovirus (CMV) promoter or the lysozyme gene regulatory domain in transiently transfected chicken blastodermal cells (CBCs). Concentration of GFP fluorescence around the periphery of these transfected CBCs suggested that GFP was being actively secreted from these cells. Furthermore, a generic lysozyme gene regulatory scaffold system has been developed by gene targeting that enables the rapid insertion of exogenous gene sequences into the chicken lysozyme gene domain, 3' of the signal peptide encoding sequence. This system will facilitate research to further test the ability of chicken lysozyme gene regulatory sequences to mediate the secretion of foreign proteins.en_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectchicken lysozyme geneen_US
dc.subjectregulatory sequencesen_US
dc.subjectgreen fluorescent proteinen_US
dc.subjectcytomegalovirus promoteren_US
dc.subjectchicken blastodermal cellsen_US
dc.titleExpression of foreign gene sequences mediated by chicken lysozyme gene regulatory sequencesen_US


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