The Isolation and Proteomic Analysis of Mycobacterium avium subspecies paratuberculosis Membrane Vesicles

dc.contributor.advisorMutharia, Lucy
dc.contributor.authorMartin, William S of Molecular and Cellular Biologyen_US of Guelphen_US of Scienceen_US and Cellular Biologyen_US
dc.description.abstractMycobacterium avium subsp. paratuberculosis (MAP) parasitizes macrophages of ruminant animals, ensuring its survival by blocking phagosome maturation and the induction of antimicrobial defences. Numerous secreted proteins have been implicated in subverting macrophage defences, however the mechanisms transporting these components are undefined. This research investigated the composition of MAP membrane vesicles (MVs) and identified MV protein composition, and antigenic MV proteins. Transmission electron microscopy visualized membrane vesicles that ranged from 20 to 270 nm in size, and immunoblotting with serum antibodies from MAP infected cattle identified seven antigenic proteins including previously identified secreted virulence factors of MAP. Collectively this work showed MVs of MAP contain proteins derived from the cell membrane, cytosol, and secreted proteins. As MAP MVs are secreted and can interact with host immune response cells, they may of be value in the search for MAP-specific epitopes for use in the detection of animals sub-clinically infected with MAP.en_US
dc.description.sponsorshipBeef Cattle Research Council
dc.description.sponsorshipGrowing Forward 2
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectMembrane Vesiclesen_US
dc.subjectJohne's diseaseen_US
dc.subjectSecreted proteinsen_US
dc.titleThe Isolation and Proteomic Analysis of Mycobacterium avium subspecies paratuberculosis Membrane Vesiclesen_US


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