The pH-activated trigger mechanism of colicin E1 channel domain

dc.contributor.advisorMerrill, A.R.
dc.contributor.authorMusse, Abdiwahab Abdul
dc.date.accessioned2020-12-04T15:02:33Z
dc.date.available2020-12-04T15:02:33Z
dc.date.copyright2002
dc.degree.departmentDepartment of Chemistry and Biochemistryen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractThe pH-trigger mechanism of colicin E1 channel peptide, previously proposed by our laboratory (Merrill 'et al.', 1997), was confirmed through the design of mutant proteins with altered pH profiles for membrane binding, insertion and activity. These mutant proteins featured cumulative substitutions of serine for the acidic residues, D-408, D-410, and D-423, that were earlier described as the principal determinant of the low pH-induced helix-to-coil conformational transition of the helix-turn-helix motif containing helices 4 and 5a. The activating helix-to-coil transition of this motif was previously probed by the low pH sensitive fluorescence decay and quantum yield parameters of the two single tryptophan mutant channel peptides, F413W and W424. In this study, the time-resolved and steady-state fluorescence behaviour of F413W single tryptophan mutants, featuring the double aspartateto-serine substitutions, D408S/D423S, indicated a random-coil-like conformational state for helix 4 of this mutant channel peptide at near neutral pH. These observations provide structural and functional credence to the proposed pH-trigger mechanism and highlight a possible role for the site-specific protonation of D-408, D-410 and D-423 in the 'in vivo' mechanism of action of pore-forming colicins.en_US
dc.identifier.urihttps://hdl.handle.net/10214/22344
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectpH-activateden_US
dc.subjecttrigger mechanismen_US
dc.subjectcolicin e1en_US
dc.subjectchannel peptideen_US
dc.subjectmutant proteinsen_US
dc.titleThe pH-activated trigger mechanism of colicin E1 channel domainen_US
dc.typeThesisen_US

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