The Creation of a Phytoglycogen-Functionalized SPR Sensor Surface and the Binding of Concanavalin A to Phytoglycogen

dc.contributor.advisorDutcher, John
dc.contributor.authorCharlesworth, Kathleen
dc.date.accessioned2019-08-21T17:41:48Z
dc.date.available2019-08-21T17:41:48Z
dc.date.copyright2019-08
dc.date.created2019-08-16
dc.date.issued2019-08-21
dc.degree.departmentDepartment of Physicsen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmePhysicsen_US
dc.description.abstractPhytoglycogen is a glucose polymer that occurs naturally in the form of highly branched, compact nanoparticles. Because of their tree-like or dendrimeric structure, phytoglycogen nanoparticles have unique properties, such as a strong interaction with water, which makes them attractive for use in applications ranging from cosmetics to drug delivery. Many of these applications rely on the binding of small molecules onto phytoglycogen nanoparticles. Surface Plasmon Resonance (SPR) is a sensitive experimental technique, based on the resonant absorption of light within an ultrathin gold film, that can be used to measure the binding kinetics and affinities of small molecules. We have successfully created a stable phytoglycogen-functionalized gold surface, using 4-Mercaptophenylboronic Acid as a linker between the gold layer and phytoglycogen. This has allowed us to use SPR to measure the association constant between phytoglycogen and Concanavalin A to be 2.87±0.44×10^5 M-1 using the Langmuir adsorption model fit.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada CGS-M
dc.identifier.urihttp://hdl.handle.net/10214/16929
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectphysicsen_US
dc.subjectbiophysicsen_US
dc.subjectsurface plasmon resonanceen_US
dc.subjectconcanavalin Aen_US
dc.subjectphytoglycogenen_US
dc.subjectassociation constanten_US
dc.subjectpolymeren_US
dc.subjectbioactiveen_US
dc.titleThe Creation of a Phytoglycogen-Functionalized SPR Sensor Surface and the Binding of Concanavalin A to Phytoglycogenen_US
dc.typeThesisen_US

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