The development of a Niemann-Pick gene-based marker in common bean (Phaseolus vulgaris L.) for the selection of common bacterial blight resistance

dc.contributor.advisorPauls, K. Peter
dc.contributor.authorMorneau, Emily
dc.date.accessioned2019-09-12T16:31:42Z
dc.date.available2019-09-12T16:31:42Z
dc.date.copyright2019-09
dc.date.created2019-09-09
dc.date.issued2019-09-12
dc.degree.departmentDepartment of Plant Agricultureen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmePlant Agricultureen_US
dc.description.abstractCommon bacterial blight (CBB) is a major disease in common bean (Phaseolus vulgaris) caused by Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans. By characterizing alleles in three candidate genes within white bean line OAC Rex, a codominant molecular marker for resistance was created. The Niemann-Pick polymorphism (NPP) marker was developed to screen common beans for CBB resistance, and is anchored in a candidate resistance gene (Niemann-Pick like gene). Individuals in two segregating populations were screened for allelic state and disease susceptibility. It was found that the NPP marker is significantly more efficient (8% decrease in error rate) in comparison to other markers. Within the OAC Rex pedigree, the resistant allele for the NPP marker was found solely within progeny from the introgression of Phaseolus acutifolius into P. vulgaris. Lastly, the two additional candidate resistance genes investigated (R-genes 231733-8-004 and 231733-8-005), were found to have no exploitable polymorphisms.en_US
dc.identifier.urihttp://hdl.handle.net/10214/17456
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectcommon beanen_US
dc.subjectcommon bacterial blighten_US
dc.subjectmolecular markeren_US
dc.subjectdisease resistanceen_US
dc.titleThe development of a Niemann-Pick gene-based marker in common bean (Phaseolus vulgaris L.) for the selection of common bacterial blight resistanceen_US
dc.typeThesisen_US

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