Alpha-galactosidase and beta-mannoside mannohydrolase in tomato seeds (Lycopersicon esculentum Mill. cv. glamour and fruit (L. esculentum Mill. cv. Trust)

dc.contributor.advisorBewley, J. Derek
dc.contributor.advisorRauser, Wilfried E.
dc.contributor.authorFeurtado, J. Allan
dc.date.accessioned2020-12-03T14:29:49Z
dc.date.available2020-12-03T14:29:49Z
dc.date.copyright1999
dc.degree.departmentDepartment of Botonyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractMannans (possibly galacto- or galactogluco-mannans) are present in tomato endosperm cell walls. Three enzymes participate in the complete depolymerization of galactomannan: [alpha]-galactosidase (EC 3.2.1.22), endo-[beta]-mannanase (EC 3.2.1.78), and [beta]-mannosidase (EC 3.2.1.25). The activity of each enzyme in tomato seeds has its own distinct program of regulation and no co-ordination of activity is seen. [alpha]-Galactosidase and [beta]-mannoside mannohydrolase activities are also present in tomato fruit pericarp and also show different patterns of activity. A cDNA encoding an [alpha]-galactosidase has been isolated from a germinated tomato seed cDNA library and shows significant homology with known dicotyledonous [alpha]-galactosidase cDNAs. [alpha]-Galactosidase gene expression is relatively constant throughout germination with a slight increase after germination. A southern hybridization analysis reveals that there is only one gene for [alpha]-galactosidase in the tomato (cv. Trust).en_US
dc.identifier.urihttps://hdl.handle.net/10214/21431
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectalpha-galactosidaseen_US
dc.subjectbeta-mannoside mannohydrolaseen_US
dc.subjecttomato seedsen_US
dc.subjectdepolymerizationen_US
dc.subjectcDNA encodingen_US
dc.titleAlpha-galactosidase and beta-mannoside mannohydrolase in tomato seeds (Lycopersicon esculentum Mill. cv. glamour and fruit (L. esculentum Mill. cv. Trust)en_US
dc.typeThesisen_US

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