Zebrafish as a tool to assess Pseudomonas aeruginosa microcolony formation in vivo

dc.contributor.advisorKhursigara, Cezar
dc.contributor.authorRocker, Adam
dc.date.accessioned2014-09-19T15:01:50Z
dc.date.available2014-09-19T15:01:50Z
dc.date.copyright2014-09
dc.date.created2014-09-17
dc.date.issued2014-09-19
dc.degree.departmentDepartment of Molecular and Cellular Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeMolecular and Cellular Biologyen_US
dc.description.abstractP. aeruginosa is commonly associated with infections in individuals with compromised host-defenses. Critical to persistence within the hostile host environment is the ability to form a biofilm, consisting of a microbial community enmeshed in extracellular polymeric substances. Much of our understanding regarding biofilm has been acquired via confocal microscopic monitoring of flow cell cultures. However, the relevance of these in vitro studies to the complex host environment remains uncertain due to the lack of a vertebral animal model amenable to such detailed visual analyses. This thesis describes the development of a larval zebrafish hindbrain ventricle infection model for the analysis of biofilm formation in vivo. Infection with wild-type P. aeruginosa PAO1 was compared with that of a psl mutant (Δpsl promoter), incapable of producing the critical biofilm exopolysaccharide Psl. Δpsl PAO1 was found to be less fatal, to produce fewer and smaller microcolonies, and to persist poorly throughout infection in comparison to the wild type. The unprecedented microcolony quantification achieved herein is dependent on the optical transparency of the zebrafish, thus demonstrating the power of the zebrafish as a tool for in vivo microcolony analyses.en_US
dc.identifier.urihttp://hdl.handle.net/10214/8477
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectmicrocolonyen_US
dc.subjectbiofilmen_US
dc.subjectpseudomonas aeruginosaen_US
dc.subjectPAO1en_US
dc.subjectzebrafishen_US
dc.subjectexopolysaccharideen_US
dc.subjectpslen_US
dc.titleZebrafish as a tool to assess Pseudomonas aeruginosa microcolony formation in vivoen_US
dc.typeThesisen_US

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