Estrogen and glucocorticoid receptor interactions on aspects of early development of rainbow trout

dc.contributor.advisorLeatherland, J.F.
dc.contributor.authorFerris, Jacqueline
dc.date.accessioned2020-12-03T14:29:49Z
dc.date.available2020-12-03T14:29:49Z
dc.date.copyright2011
dc.degree.departmentDepartment of Biomedical Sciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractThis thesis is an investigation of the interactions between the estrogen (ER) and glucocorticoid (GR) receptors. The study examined the effects of ' in ovo' exposure of rainbow trout to GR and ER agonists (Cortisol and Bisphenol A [BPA], respectively) and antagonists (RU-486 and ICI 182,780, respectively) in various chemical combinations on mRNA transcription of 'ER[alpha], ER[beta], GR1' and 'GR2'. BPA did not affect mRNA transcription of 'ER[alpha]' or ' ER[beta]', and exhibited differential effects on 'GR1' and 'GR'2, depending on the time point at which gene transcription was analyzed. The antiestrogen ICI 182,780 did not act as a pure antiestrogen, but acted differentially on 'ER[alpha]' and 'ER[beta] '. Histological analysis revealed co-administration of cortisol and BPA 'in ovo' caused marked effects on notochord development indicated by an absence of the chordoblast layer. 'In ovo' exposure to toxicants can affect early epigenetic mechanisms, possibly leading to deformity and/or death of a developing rainbow trout embryo.en_US
dc.identifier.urihttps://hdl.handle.net/10214/21430
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectestrogenen_US
dc.subjectglucocorticoiden_US
dc.subjectreceptorsen_US
dc.subjectin ovo exposureen_US
dc.subjectrainbow trouten_US
dc.subjectagonistsen_US
dc.subjectantagonistsen_US
dc.subjectmRNA transcriptionen_US
dc.subjectER[alpha]en_US
dc.subjectER[beta]en_US
dc.subjectGR1en_US
dc.subjectGR2en_US
dc.titleEstrogen and glucocorticoid receptor interactions on aspects of early development of rainbow trouten_US
dc.typeThesisen_US

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