Selection and characterization of alpha-cobrotoxin specific llama single-domain antibodies

dc.contributor.advisorHall, J. Christopher
dc.contributor.authorStewart, Christine S.
dc.date.accessioned2021-04-28T16:02:26Z
dc.date.available2021-04-28T16:02:26Z
dc.date.copyright2006
dc.degree.departmentDepartment of Environmental Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractSnake bite is a serious global medical concern for which the only effective treatment is the administration of specific antivenom. Conventional antivenoms, however, often elicit adverse reactions and have mismatched pharmacokinetic profiles with their target toxins due to antibody/toxin size discrepancies. VHHs, the smallest antigen binding fragment, are highly diffusible, stable under physiological conditions, non-immunogenic, and are easily produced ' in vitro' using bacterial and yeast expression systems. Three [alpha]-cobrotoxin specific VHHs were isolated from a nai?ve llama phage display library. [alpha]-Cobrotoxin binding specificity was determined via phage-displayed VHH ELISA. Surface plasmon resonance analysis determined the K D values to be 2, 3 and 3 [mu]M; however, these affinities were too low for [alpha]-cobrotoxin detection by soluble VHH ELISA. A pentamerization strategy, linking the VHH to the verotoxin 1 B subunit, improved the VHH functional affinities for ELISA development. VHH-based antivenoms may improve antivenom biodistribution and neutralization while also reducing adverse effects.en_US
dc.identifier.urihttps://hdl.handle.net/10214/25590
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectalpha-cobrotoxinen_US
dc.subjectllama single-domain antibodiesen_US
dc.subjectVHH-based antivenomsen_US
dc.subjectisolationen_US
dc.subjectdetectionen_US
dc.subjectVHH ELISAen_US
dc.titleSelection and characterization of alpha-cobrotoxin specific llama single-domain antibodiesen_US
dc.typeThesisen_US

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