Successful Cryopreservation of Asian elephant (Elephas maximus) semen using simple techniques

dc.contributor.advisorBédécarrats, Gregoy
dc.contributor.authorArnold, Danielle
dc.date.accessioned2014-05-06T15:38:22Z
dc.date.available2014-05-06T15:38:22Z
dc.date.copyright2014-04
dc.date.created2014-04-30
dc.date.issued2014-05-06
dc.degree.departmentDepartment of Animal and Poultry Scienceen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeAnimal and Poultry Scienceen_US
dc.description.abstractThis thesis is an investigation of the effect of simple freezing techniques, storage at 4°C and different thaw temperatures on post-thaw measures of cryopreserved Asian elephant semen samples. There was a significant effect of freeze rate on post-thaw motility (P<0.0001) and acrosomal integrity (P<0.005) of straws frozen after 0 and 24 hr storage at 4°C. The faster freeze rates in the dry shipper and at 1cm or 2 cm above liquid nitrogen consistently provided better cryopreservation than slower freezing rates. Thaw temperature had no effect on post-thaw semen quality. Storage of samples prior to freezing had a detrimental effect on post-thaw semen quality. Placing straws directly in a dry shipper to freeze and thawing them at 50°C was a simple technique for effectively cryopreserving Asian elephant semen.en_US
dc.description.sponsorshipInternational Elephant Foundation
dc.identifier.urihttp://hdl.handle.net/10214/8063
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectelephanten_US
dc.subjectsemenen_US
dc.subjectcryopreservationen_US
dc.subjectfreezingen_US
dc.subjectthawingen_US
dc.subjectreproductionen_US
dc.subjectdry shipperen_US
dc.subjectliquid nitrogenen_US
dc.titleSuccessful Cryopreservation of Asian elephant (Elephas maximus) semen using simple techniquesen_US
dc.typeThesisen_US

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