The use of Potentially Probiotic Bacteria for their Biocontrol Effects on the Foodborne Pathogen Human Norovirus GII.4 and its Surrogate Virus Murine Norovirus-1

dc.contributor.advisorFarber, Jeffrey
dc.contributor.authorDallner, Mattthew
dc.date.accessioned2020-09-30T20:45:20Z
dc.date.available2020-09-30T20:45:20Z
dc.date.copyright2020-09
dc.date.created2020-09-25
dc.degree.departmentDepartment of Food Scienceen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeFood Scienceen_US
dc.description.abstractThis thesis is an investigation of potential probiotic bacteria for their biocontrol effects against the foodborne pathogen human norovirus (HuNoV) and its surrogate murine norovirus-1 (MNV-1). Norovirus (NoV) is the foodborne pathogen responsible for the largest number of foodborne illnesses worldwide, but there is a noticeable lack of therapeutics available. In in vitro infectivity experiments no significant increases in BV-2 survival were observed after potential probiotic treatment. The next objective was to asses the ability of six selected potential probiotics to bind to both viruses measured using droplet digital PCR (ddPCR). Lactobacillus rhamnosus was the only strain to cause a statistically significant reduction in MNV-1 genome copies/mL, indicating that it may be able to bind to MNV-1. For HuNoV GII.4, none of the probiotics caused a significant reduction, and only E. cloacae, the binding control, did. This work gives further evidence of bacteria-virus interactions, but more study is required.en_US
dc.description.sponsorshipCanada First Research Excellence Fund
dc.identifier.urihttps://hdl.handle.net/10214/21322
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectNorovirusen_US
dc.subjectProbiotic bacteriaen_US
dc.subjectBindingen_US
dc.subjectddPCRen_US
dc.subjectLactobacillusen_US
dc.subjectMurine norovirusen_US
dc.titleThe use of Potentially Probiotic Bacteria for their Biocontrol Effects on the Foodborne Pathogen Human Norovirus GII.4 and its Surrogate Virus Murine Norovirus-1en_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Dallner_Matthew_202009_MSc.pdf
Size:
6.22 MB
Format:
Adobe Portable Document Format
Description:
Thesis - Full