Production of Recombinant Human Butyrylcholinesterase in Nicotiana benthamiana

dc.contributor.advisorHall, Christopher J.
dc.contributor.authorHayward, Robin L.
dc.date.accessioned2012-10-03T18:30:58Z
dc.date.available2012-10-03T18:30:58Z
dc.date.copyright2012-09
dc.date.created2012-09-24
dc.date.issued2012-10-03
dc.degree.departmentDepartment of Environmental Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeEnvironmental Biologyen_US
dc.description.abstractNerve agents (NAs) inhibit the essential enzyme acetylcholinesterase. Classified as chemical weapons, NAs are considered a threat to soldiers on the frontlines of warzones. Current treatments can prevent death from NA poisoning, but are not effective in preventing convulsions, seizures, or subsequent brain damage. Butyrylcholinesterase (BChE) binds to NAs, rendering the chemicals harmless to acetylcholinesterase.. Two hundred mg of BChE is the putative prophylactic dose for adult humans, but is difficult to obtain in large quantities from expired human serum. Although recombinant BChE has been expressed in several organisms, the yields are still low. Nicotiana benthamiana is an attractive plant for transient protein production due to its quick growth rate, abundance of tissue, and history of successful recombinant protein production. For this research, N. benthamiana was infiltrated with viral based vectors as well as binary vectors containing the human BChE gene. Multiple assays indicated that binary vector BChE-105-1 + P19 enabled the best expression, producing 26 mg BChE/kg tissue.en_US
dc.description.sponsorshipCanada Research Chair Program
dc.description.sponsorshipOntario Ministry of Agriculture, Food and Rural Affairs
dc.identifier.urihttp://hdl.handle.net/10214/4038
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectButyrylcholinesteraseen_US
dc.subjectNerve Agentsen_US
dc.subjectOrganophosphatesen_US
dc.subjectNicotiana benthamianaen_US
dc.subjectRecombinant Proteinen_US
dc.titleProduction of Recombinant Human Butyrylcholinesterase in Nicotiana benthamianaen_US
dc.typeThesisen_US

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