Quantifying Spatiotemporal Patterns in the Expansion of Twitching Bacterial Colonies

dc.contributor.advisorDutcher, John
dc.contributor.authorShelton, Erin
dc.date.accessioned2015-09-08T18:58:12Z
dc.date.available2015-09-08T18:58:12Z
dc.date.copyright2015-08
dc.date.created2015-08-31
dc.date.issued2015-09-08
dc.degree.departmentDepartment of Physicsen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeBiophysicsen_US
dc.description.abstractType IV pili (T4P) are protein filaments used by certain Gram-negative bacteria for a mode of surface motility called twitching. We have developed experimental and data analysis techniques to quantify the expansion of twitching P. aeruginosa colonies at an agar-glass interface as a function of agar concentration C. The advancing front consists of finger-like protrusions of bacteria, with cells in the expanding colony arranged in a lattice-like pattern. We find that the average finger width increases linearly with C, whereas the average finger speed is independent of C. This latter result, coupled with the observed transition from monolayer to multilayer coverage within fingers, suggests a critical number of T4P are required to break through the agar-glass interface. In addition, we have calculated line profiles across fingers, quantifying their average speed and bacterial orientation, and performed high spatial and temporal resolution studies of the fingers.en_US
dc.identifier.urihttp://hdl.handle.net/10214/9166
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectcollective motionen_US
dc.subjectbacterial twitching motilityen_US
dc.subjecttype iv pilien_US
dc.subjectagar stiffnessen_US
dc.titleQuantifying Spatiotemporal Patterns in the Expansion of Twitching Bacterial Coloniesen_US
dc.typeThesisen_US

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