A Structural and Functional Investigation of Reptilian Skin Using the Leopard Gecko (Eublepharis macularius)

dc.contributor.advisorVickaryous, Matthew
dc.contributor.authorSchwann, Keeley
dc.date.accessioned2023-05-19T19:36:08Z
dc.date.available2023-05-19T19:36:08Z
dc.date.copyright2023-05
dc.date.created2023-05-16
dc.degree.departmentDepartment of Biomedical Sciencesen_US
dc.degree.grantorUniversity of Guelphen
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeRegenerative Medicineen_US
dc.description.abstractSkin is a bi-layered organ that heals either through scar formation or scar-free regeneration. Using the leopard gecko, a species capable of spontaneous regeneration, we conducted a structural and functional investigation of skin before, during and after regeneration. Using ImageJ/FIJI, we quantified various measurements of vascularity and collagen architecture from skin of the original body, original tail, and regenerated tail. We also used uniaxial and biaxial tensile testing to explore skin function. Overall, we determined that the vascularity and collagen architecture of regenerated skin is structurally equivalent to original skin. With uniaxial testing, we found that the tensile strength of regenerated skin improves over time, but it never achieves pre-injury values. Using biaxial testing, we determined that the tensile properties of original skin varied across the body and during ontogeny. Taken together, these data underscore the complexity of skin and suggest structural but not functional replacement is achieved during regeneration.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.identifier.urihttps://hdl.handle.net/10214/27638
dc.language.isoenen_US
dc.publisherUniversity of Guelphen
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjecttensile testingen_US
dc.subjectregenerationen_US
dc.subjectskinen_US
dc.subjectblood vesselsen_US
dc.subjectcollagenen_US
dc.subjectuniaxialen_US
dc.subjectleopard geckoen_US
dc.subjectlizarden_US
dc.subjectwound healingen_US
dc.subjectbiomechanical testingen_US
dc.subjectontogenyen_US
dc.subjectquantificationen_US
dc.subjectImageJ/Fijien_US
dc.subjectbiaxialen_US
dc.titleA Structural and Functional Investigation of Reptilian Skin Using the Leopard Gecko (Eublepharis macularius)en_US
dc.typeThesisen

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