Genetic Study and QTL Analysis on Soybean Seed Protein Quality and Quantity

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dc.contributor.advisorEskandari, Milad
dc.contributor.authorZhang, Jianyu
dc.date.accessioned2022-12-22T16:34:11Z
dc.date.available2022-12-22T16:34:11Z
dc.date.copyright2022-12
dc.date.created2022-12-13
dc.degree.departmentDepartment of Plant Agricultureen_US
dc.degree.grantorUniversity of Guelphen
dc.degree.nameMaster of Scienceen_US
dc.degree.programmePlant Agricultureen_US
dc.description.abstractSoybean (Glycine max (L.) Merrill) protein quality is limited by deficiencies in the concentrations of amino acids cysteine (Cys), methionine (Met), threonine (Thr), and lysine (Lys). This study aimed to: (1) assess the effects of genotype, environment, and genotype-by-environment interactions (GEI) on seed protein quantity and quality in two recombinant inbred line (RIL) populations derived from amino acid manipulated fast neutron mutants, in multi-environment trials (MET) conducted in four environments in southern Ontario; and (2) to identify and validate quantitative trait loci (QTL) associated with seed protein quantity and quality in the two populations. Genotype and environment effects were significant for all target traits. Significant GEI effects on protein, Thr, and Lys were observed in at least one population. A modified QTL-seq analysis discovered genomic regions associated with target traits in three of the 20 soybean chromosomes. These results may be useful for breeding efforts on improving soybean protein nutrition.en_US
dc.description.sponsorshipGrain Farmers of Ontario
dc.description.sponsorshipSeCan
dc.description.sponsorshipMitacs
dc.identifier.urihttps://hdl.handle.net/10214/27345
dc.language.isoenen_US
dc.publisherUniversity of Guelphen
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectsoybeanen_US
dc.subjectquantitative trait locien_US
dc.subjectQTLen_US
dc.subjectgenotype by environment interactionen_US
dc.subjectQTL-seqen_US
dc.subjectsoybean proteinen_US
dc.subjectsoybean cysteineen_US
dc.subjectsoybean methionineen_US
dc.subjectsoybean threonineen_US
dc.subjectsoybean lysineen_US
dc.titleGenetic Study and QTL Analysis on Soybean Seed Protein Quality and Quantityen_US
dc.typeThesisen
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