Effect of kappa-carrageenan on phase separation of milk proteins and polysaccharide stabilizer in sucrose solution simulating ice cream mix system

dc.contributor.advisorGoff, H. Douglas
dc.contributor.authorThaiudom, Sukrit
dc.date.accessioned2021-04-26T19:47:33Z
dc.date.available2021-04-26T19:47:33Z
dc.date.copyright2001
dc.degree.departmentDepartment of Food Scienceen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameDoctor of Philosophyen_US
dc.description.abstractMilk proteins and polysaccharide stabilizers commonly exhibit phase separation when they are mixed. Practical experience has shown that [kappa]-carrageenan can prevent phase separation. However, not much research has been conducted on the quaternary system of [kappa]-carrageenan/primary stabilizer/milk protein/sucrose solution, especially rheological properties. The effects of heat treatment, homogenization, and butteroil, added to simulate ice cream mix, on rheological properties were studied using a dynamic controlled stress rheometer. Phase diagrams and transmission electron microscopy (TEM) were used to investigate structural formation at the macroscopic and microscopic levels, respectively. Gel electrophoresis was used to analyze milk protein surface concentration in systems with butteroil. Disappearance of phase separation between milk proteins and primary stabilizers in sucrose solution at the macroscopic level was evident with added [kappa]-carrageenan but the existence of phase separation at the microscopic level was still present. Phase separation was attributed to a depletion flocculation mechanism while disappearance of this phenomenon was attributed either to a weak filament gel-network of [kappa]-carrageenan, adsorbed on caseins via electrostatic interaction, or to a gelation of excess [kappa]-carrageenan itself. Pseudoplastic behaviour and elastic-like characteristics were more evident with increasing concentration of [kappa]-carrageenan in all quaternary systems with different types of primary stabilizers. Heat treatment effectively increased thixotropic characteristics of these quaternary systems. Homogenization possibly reduced chain length of LBG, xanthan, and [kappa]-carrageenan, leading to less self-association of xanthan but more synergistic effect between LBG and [kappa]-carrageenan. This resulted in less elastic-like behaviour in the former and less viscous behaviour in the latter, as demonstrated by a higher tan [delta] and a lower tan [delta], respectively. Such structural characteristics were shown by TEM. When adding butteroil, thixotropic characteristics were more evident in systems comprised of xanthan than LBG systems. However, the more [kappa]-carrageenan added into systems with butteroil, the more solid-like characteristic. The results from SDS-PAGE showed that LBG or xanthan with or without [kappa]-carrageenan significantly affected milk protein concentration on the butteroil droplet surface.en_US
dc.identifier.urihttps://hdl.handle.net/10214/25442
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectheat treatmenten_US
dc.subjecthomogenizationen_US
dc.subjectbutteroilen_US
dc.subjectice cream mix systemen_US
dc.subjectrheological propertiesen_US
dc.subjectdynamic controlled stress rheometeren_US
dc.subjectkappa-carrageenanen_US
dc.subjectphase separatioen_US
dc.subjectmilk proteinsen_US
dc.subjectpolysaccharide stabilizeren_US
dc.subjectsucrose solutionen_US
dc.titleEffect of kappa-carrageenan on phase separation of milk proteins and polysaccharide stabilizer in sucrose solution simulating ice cream mix systemen_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Thaiudom_Sukrit_PhD.pdf
Size:
9.95 MB
Format:
Adobe Portable Document Format