Thrombospondin expression localized to granulosa cells in the bovine ovary

dc.contributor.advisorGentry, P.A.
dc.contributor.advisorLaMarre, J.
dc.contributor.authorBond, Jennifer Elizabeth of Biomedical Sciencesen_US of Guelphen_US of Scienceen_US
dc.description.abstractThrombospondin is a 450 kDa glycoprotein first identified as a secretory product of thrombin-stimulated platelets. Recently, components of the thrombin-generating pathway of hemostasis have been identified in follicular fluid. Endogenously synthesized thrombospondin is found in the extracellular matrix of hormone responsive cells, such as bovine adrenocortical cells, and has been demonstrated to be influential in regulating their morphology. Western blot analysis of follicular fluid and granulosa cell lysates, isolated from bovine ovaries collected at slaughter, detected the presence of thrombospondin. Northern blot analysis of total RNA from granulosa cells demonstrated that thrombospondin 1 and thrombospondin 2 mRNA is present at highest levels in the early stages of follicular organization. These studies reveal that thrombospondin is produced by granulosa cells and secreted into the surrounding follicular fluid in decreasing concentrations as the follicle size increases, supporting a potential role for thrombospondin in bovine follicular development.en_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectBovine ovaryen_US
dc.subjectGranulosa cellsen_US
dc.subjectFollicular fluiden_US
dc.subjectThrombin-stimulated plateletsen_US
dc.titleThrombospondin expression localized to granulosa cells in the bovine ovaryen_US


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