The detection of foodborne pathogens using bacteriophage-based AK assay

dc.contributor.advisorGriffiths, M.W.
dc.contributor.authorWu, Yanli
dc.date.accessioned2021-04-16T18:16:06Z
dc.date.available2021-04-16T18:16:06Z
dc.date.copyright2000
dc.degree.departmentDepartment of Food Scienceen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractA sensitive and rapid phage-mediated AK assay was developed for the detection of 'E. coli' G2-2 and 'S. enteritidis '. In this assay, a bacteriophage is employed as a lytic agent for its target bacteria. The number of bacterial cells presence can be detected by measuring the degree of lysis and hence the release of intracellular adenylate kinase (AK). AK is assayed in the presence of excess ADP by measuring the formation of ATP by bioluminescence using the luciferase/luciferin reaction. In this study, the commercial ADP was purified for the assay using HPLC technique. For 'E. coli' G2-2 and 'S. enteritidis', the detection limits in pure culture were estimated to be approximately 1.2 * 103 CFU/ml and 1.5 * 103 CFU/ml, respectively. The effect of competitive flora on the ability of phages to infect host cells was also evaluated. The detection limit of 'E. coli' G2-2 in mixed culture was around 1.4 * 103 CFU/ml; for ' S. enteritidis', it was approximately 1.7 * 103 CFU/ml.en_US
dc.identifier.urihttps://hdl.handle.net/10214/24958
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectsensitiveen_US
dc.subjectrapiden_US
dc.subjectphage-mediated AK assayen_US
dc.subjectdetectionen_US
dc.subjectE. colien_US
dc.subjectS. enteritidisen_US
dc.subjectbacteriophageen_US
dc.subjectadenylate kinaseen_US
dc.titleThe detection of foodborne pathogens using bacteriophage-based AK assayen_US
dc.typeThesisen_US

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