Identification and characterization of species-specific lactic acid bacteria for potential probiotic use in cattle

dc.contributor.advisorStaempfli, Henry
dc.contributor.advisorWeese, J. Scott
dc.contributor.authorRodriguez-Palacios, Alexander of Clinical Studiesen_US of Guelphen_US of Scienceen_US
dc.description.abstractThis study investigated lactic acid bacteria (LAB) isolated from bovine samples, and evaluated the colonization ability, fecal recovery and safety of one isolate with probiotic properties in a 15-day blind placebo study in calves. 104 LAB were assessed 'in-vitro' for resistance to low pH, bile salts, aerobic incubation, and antimicrobial activity against ' Escherichia coli' F5 and O157. Fourteen and 42 percent of the isolates had appropriate overall tolerance (growth rate >80% compared to controls) to pH 4 and 0.3% bile. The inhibitory effect of LAB supernatants on ' E. coli' was associated to their low pH (r2 = 0.804, ' p' < 0.001). The selected isolate (LPB80) was identified as ' Lactobacillus plantarum' by 16S rRNA-PCR. LPB80 administration had no adverse effects. LPB80 was recovered from feces of treated and non-treated calves and from intestinal samples of treated calves. Results suggested that oral administration of LPB80 is safe and that LPB80 may colonize the intestinal tract of calves.en_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectprobiotic useen_US
dc.subjectlactic acid bacteriaen_US
dc.subjectcolonization abilityen_US
dc.subjectfecal recoveryen_US
dc.titleIdentification and characterization of species-specific lactic acid bacteria for potential probiotic use in cattleen_US


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