The Role of the C-terminal Coiled-coil in the Cellular Localization and in the Osmotic Stress Response of Transporter ProP

dc.contributor.advisorWood, Janet
dc.contributor.authorMarom, David
dc.date.accessioned2015-05-06T18:57:15Z
dc.date.available2015-05-06T18:57:15Z
dc.date.copyright2015-05
dc.date.created2015-05-04
dc.date.issued2015-05-06
dc.degree.departmentDepartment of Molecular and Cellular Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeMolecular and Cellular Biologyen_US
dc.description.abstractProP of Escherichia coli (ProPEc) is an osmosensory transporter and an H+-osmolyte symporter. Disruption of the antiparallel coiled-coils of ProPEc and Agrobacterium tumefaciens ProP (ProPAt) elevated the osmolalities at which the transporters became active. Corynebacterium glutamicum ProP (ProPCg), which lacks the coiled-coil heptads, required a higher osmolality for activation than ProPEc and ProPAt. In this work, the ProP orthologue from Xanthomonas campestris (ProPXc) was expressed and characterized in E. coli. ProPXc lacks coiled-coils heptads and has higher sequence identity to ProPEc (51%) than ProPCg (37%). ProPXc was found active at a lower osmolality than ProPEc, which contradicts the previously established pattern. Previously, fluorophore-tagged ProPEc was found to localize in E. coli cells’ poles and septa in correlation to the proportion of phospholipid cardiolipin (CL). To corroborate these findings, GFPmut2 was fused to ProPEc. In this work, we found some indications of correlation between the localization pattern of ProPmut2A206K-ProPEc and CL.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.identifier.urihttp://hdl.handle.net/10214/8833
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectosmosensoren_US
dc.subjectosmoregulatoryen_US
dc.subjecttransporteren_US
dc.subjectProPen_US
dc.titleThe Role of the C-terminal Coiled-coil in the Cellular Localization and in the Osmotic Stress Response of Transporter ProPen_US
dc.typeThesisen_US

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