The classification and evolution of Pokey, a DNA transposon in Daphnia

dc.contributor.advisorCrease, T.J.
dc.contributor.authorPenton, Erin Heather of Zoologyen_US of Guelphen_US of Scienceen_US
dc.description.abstractThe recent explosion of newly reported eukaryotic transposable elements is illustrating their widespread nature, their ultimate importance as agents of evolution, and the genome as a dynamic entity. My thesis characterizes a transposable element, 'Pokey', found in the ribosomal DNA of 'Daphnia'. Through sequencing of full-length 'Pokey ' elements from 'Daphnia pulicaria', I have shown that it is related to 'piggyBac' and thus, is a member of the TTAA-specific family of DNA transposons. A phylogenetic analysis of the 3' -end of 'Pokey' elements from the subgenus ' Daphnia' showed that 'Pokey' persists via strict vertical transmission and has existed for long periods of evolutionary time, which is unusual for DNA transposons. 'Pokey' inserts into a region of the large subunit ribosomal RNA gene occupied by other elements and it seems likely that this region is an ideal genomic location for the evolution of insertion sequences.
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectDNA transposon
dc.subjecteukaryotic transposable elements
dc.subjectribosomal DNA
dc.titleThe classification and evolution of Pokey, a DNA transposon in Daphnia


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