Ion transport mechanism in gramicidin A channels formed in floating bilayer lipid membranes supported on gold electrodes

dc.contributor.authorSu, Z.F.
dc.contributor.authorGoodall, B.
dc.contributor.authorLeitch, J.J.
dc.contributor.authorlipkowski, J.
dc.date.accessioned2022-01-28T16:50:29Z
dc.date.available2022-01-28T16:50:29Z
dc.date.createdApr-21
dc.degree.departmentElectrochemical Technology Centreen
dc.degree.departmentDepartment of Chemistryen
dc.description.abstractThe ion channel properties of gramicidin A (gA) in a model floating bilayer lipid membrane were studied by electrochemical impedance spectroscopy (EIS) and polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS). To determine if the transport of Na+ across the membrane is coupled with the transport of the counterion, fluoride and perchlorate anions were explored due to their large differences in hydration energy. The combined EIS and IR experiments demonstrated that in contrast to valinomycin, the mechanism of Na+ transfer through the gA channel is independent of the accompanying anion present in the supporting electrolyte. These studies also demonstrated that the conductivity of the gA sodium channel depends on the transmembrane potential and the IR measurements indicated that more gA molecules insert into the bilayer with the decrease of the transmembrane potential. The results of this present study demonstrate that the fBLM system is a suitable model bilayer for the in situ investigations of membrane-active peptides.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (NSERC)en_US
dc.identifier.urihttps://hdl.handle.net/10214/26752
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectgramicidinen_US
dc.subjectEISen_US
dc.subjectPM-IRRASen_US
dc.titleIon transport mechanism in gramicidin A channels formed in floating bilayer lipid membranes supported on gold electrodesen_US
dc.typeArticleen_US
dcterms.relationZhangFei Su, Barbara Goodall, J. Jay Leitch and Jacek Lipkowski, Ion transport mechanism in gramicidin A channels formed in floating bilayer lipid membranes supported on gold electrodes, Electrochimica Acta, 2021,375,137892. https://doi.org/10.1016/j.electacta.2021.137892en_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
EA_2021_1.pdf
Size:
851.62 KB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.74 KB
Format:
Item-specific license agreed upon to submission
Description:

Collections