Characterizing bacterial diversity in undefined dairy starter cultures used for Cheddar cheese production

dc.contributor.advisorLaPointe, Gisele
dc.contributor.authorSiddiqi, Myra
dc.date.accessioned2020-08-25T13:24:13Z
dc.date.copyright2020-08
dc.date.created2020-07-09
dc.degree.departmentDepartment of Food Scienceen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeFood Scienceen_US
dc.description.abstractMesophilic mixed cultures commonly used in Cheddar cheese production contain a mix of Lactococcus lactis subspecies cremoris and subspecies lactis. To explore microbial diversity of Cheddar cheese starter mixtures, the community dynamics within nine undefined starter mixes were determined by 16S amplicon sequencing, shotgun metagenomics and L. lactis isolate whole genome sequencing. Each starter culture showed a distinctive major consensuses sequence based on 183 Lactococcus lactis species-specific markers. The genomes of 97 isolates of L. lactis subsp. cremoris were grouped into 7 clades and their functions were compared using RAST (Rapid Annotation using Subsystem Technology). Reddy differential agar was used to differentiate isolates between lactococcal subtypes, and those isolates were further classified for proteolytic and acidification performance. Isolates were grouped into categories of cremoris genotype cremoris phenotype (‘true cremoris’) and cremoris genotype lactis phenotype. This can help further explain the cremoris/lactis genotype and phenotype discrepancies of the Lactococcus lactis subspecies.en_US
dc.description.embargo2021-07-31
dc.identifier.urihttps://hdl.handle.net/10214/20646
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectDairyen_US
dc.subjectMicrobiologyen_US
dc.subjectgenomicsen_US
dc.subjectmolecular biologyen_US
dc.subjectstarter culturesen_US
dc.titleCharacterizing bacterial diversity in undefined dairy starter cultures used for Cheddar cheese productionen_US
dc.typeThesisen_US

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