Sequestration of picloram by an anti-picloram antibody to provide herbicide resistance in tobacco

dc.contributor.advisorHall, J. Christopher
dc.contributor.authorHorsman, Jonathan S.
dc.date.accessioned2020-12-03T18:09:30Z
dc.date.available2020-12-03T18:09:30Z
dc.date.copyright2006
dc.degree.departmentDepartment of Environmental Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractA single-chain variable fragment (scFv) antibody specific for the auxinic herbicide, picloram, was expressed in tobacco ('Nicotiana tabacum' L.) to determine if this antibody could attenuate the effects of the herbicide, thereby creating a new mode of developing herbicide resistant crops. Transgenic plants expressing this antibody tolerated picloram doses of 5 g ai ha-1 while wild-type plants died. Translocation of [14C]picloram from the treated leaf to the meristem was reduced in tobacco expressing the anti-picloram antibody, and more [14C]picloram was found in the roots of plants expressing the anti-picloram antibody. No significant metabolism of picloram was observed in either wildtype control plants or plants expressing the anti-picloram antibody. This research indicates that this model system may provide a relatively rapid method of engineering plants for resistance to foliar and soil applied herbicides and environmental contaminants.en_US
dc.identifier.urihttps://hdl.handle.net/10214/22015
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectsingle-chain variable fragmenten_US
dc.subjectantibodyen_US
dc.subjectauxinic herbicideen_US
dc.subjectpicloramen_US
dc.subjecttobaccoen_US
dc.subjectNicotiana tabacum L.en_US
dc.subjectattenuationen_US
dc.subjectherbicide resistant cropsen_US
dc.titleSequestration of picloram by an anti-picloram antibody to provide herbicide resistance in tobaccoen_US
dc.typeThesisen_US

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