A cytological and molecular analysis of cattle, bull clones and their offspring

dc.contributor.advisorKing, W. Allan
dc.contributor.authorHart, Ezra J.
dc.degree.departmentDepartment of Biomedical Sciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractTo assess the impact of cloning by SCNT on the meiotic processes, we undertook a molecular and immunocytological comparison of bull clones and their offspring. This was the first assessment of recombination frequency in cattle meiosis and the first attempt to examine the effect of SCNT on recombination and the associated proteins, within any cloned species to date. The recombination profile of five normal bulls of proven fertility, two SCNT-derived bulls and four mature offspring of SCNT bulls were examined through immunocytology and showed that the mean number of crossing over events per spermatocyte for SCNT-derived bulls (43 ± 5SD, ranging from 35 to 56), and their offspring (46 ± 4SD, ranging from 37 to 58) was not significantly different from normal non clone bulls (42 ± 4 SD, ranging from 33 to 56). Additionally, initial results indicate that there is a similar level of recombination in female cattle to that of the male. The mRNA expression levels of the meiotic proteins SCP3 and MLH1 were also examined, using real time quantitative PCR (RT-PCR). Again, no significant difference was observed between groups in expression levels of either protein. Lastly telomere lengths were compared between groups and no significant difference was found. It would appear that the SCNT procedure has had no influence on the meiotic processes and reproductive health of animals produced by SCNT or their offspring.en_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectsomatic cell nuclear transferen_US
dc.subjectmeiotic processesen_US
dc.subjectbull clonesen_US
dc.subjectrecombination frequencyen_US
dc.subjectreproductive healthen_US
dc.titleA cytological and molecular analysis of cattle, bull clones and their offspringen_US


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