Apple pomace polyphenols and their effect on the proliferation of human epithelial colorectal adenocarcinoma cells (HT-29)
In an effort to eventually produce a human health promoting, polyphenol-enriched dairy product for human consumption by apple pomace (AP) inclusion in the diet of a dairy cow, two preliminary studies were conducted. Initially, 8 commonly found polyphenols, in locally-sourced AP, were quantified via UPLC/MS and total polyphenol content was determined by the Fast Blue BB salt method. To determine the effect of the rumen environment on the recovery of polyphenols originally found in AP, the sample was subjected to 24hr rumen digestion in an in vitro incubator and the quantities of the polyphenols in the digesta were analyzed by UPLC/MS. The second study evaluated the effect of the aglycone (quercetin) of the most adundant polyphenol in this sample of AP, quercetin-3-glucoside, on the proliferation of human epithelial colorectal adenocarcinoma cells (HT-29). Their proliferation was quantified by use of a cell-protein binding dye, sulforhodamine B.