A cadaveric specimen was imaged using a Computed Tomography (CT) scanner under unloaded and loaded conditions using a six-degrees-of-freedom force applicator in order to provide input data for future validatable Finite Element (FE) models. An objective evaluation of different segmentation methods applied on the CT scans of the wrist was performed, and the Canny method was selected to be applied to the CT scans of the wrist. A combination of the Canny method of edge detection and a filtering process was used in the CT image segmentation procedure. A three-dimensional representation of the wrist was generated that could be used as a basis for the development of future FE models. The three-dimensional representation of the wrist under the unloaded condition may be used to construct an FE model of the wrist, whereas the CT scans of the wrist under loaded conditions may be used to validate the FE model.