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Characterization of peroxisomal multivesicular body morphology and the role of host-cell and viral components in their biogenesis in plant and yeast cells

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dc.contributor.author Gibson, Kimberley
dc.date 2009-11-30
dc.date.accessioned 2009-12-21T18:35:14Z
dc.date.available 2009-12-21T18:35:14Z
dc.date.issued 2009-12-21T18:35:14Z
dc.identifier.uri http://hdl.handle.net/10214/2100
dc.description.abstract Peroxisome biogenesis is complex, involving a diverse array of intracellular pathways and mechanisms that mediate their biogenesis and cellular functions. Relevant to our understanding of peroxisome biogenesis is the utilization of peroxisomal membranes for viral genome replication as observed in plant cells infected by several members of the Tombusviridae family of positive-strand RNA viruses. Tomato Bushy Stunt Virus (TBSV), for instance, usurps an array of host factors that facilitate the transformation of peroxisomes into peroxisomal multivesicular bodies (pMVB) the sites of viral RNA replication. In this study, pMVB topology and biogenesis was investigated using transmission electron and epifluorescence microscopy of tobacco and wildtype or mutant budding yeast that were transformed with TBSV replicase proteins and a defective interfering viral RNA. Overall, the results suggest that host-virus interactions specifically associated with Endosomal Sorting Complex Required for Transport (ESCRT) and lipid metabolism are involved in TBSV replication and pMVB biogenesis. en
dc.language.iso en en
dc.subject endosomal sorting complex required for transport en
dc.subject escrt en
dc.subject plant virus en
dc.subject host-viral interaction en
dc.subject host-virus interaction en
dc.subject pMVB en
dc.subject peroxisomal multivesicular body en
dc.subject peroxisomes en
dc.subject peroxisome biogenesis en
dc.subject tbsv en
dc.subject tomato bushy stunt virus en
dc.title Characterization of peroxisomal multivesicular body morphology and the role of host-cell and viral components in their biogenesis in plant and yeast cells en
dc.type Thesis en
dc.degree.programme Molecular and Cellular Biology en
dc.degree.name Master of Science en
dc.degree.department Department of Molecular and Cellular Biology en


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