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Establishing the Relationship Between Nitrogen Assimilation and Photoperiodic Injury in Tomato (Solanum lycopersicum L.)

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dc.contributor.advisor Micallef, Barry
dc.contributor.advisor Colasanti, Joseph
dc.contributor.advisor Raizada, Manish
dc.contributor.author Innes, Gregory
dc.date.accessioned 2018-01-05T21:02:57Z
dc.date.available 2019-01-04T06:00:19Z
dc.date.copyright 2018-01
dc.date.created 2018-01-04
dc.date.issued 2018-01-05
dc.identifier.uri http://hdl.handle.net/10214/12161
dc.description.abstract Photoperiodic injury (PI) in tomato (Solanum lycopersicum L.) is characterized by chlorosis of vegetative tissues under extended photoperiods. Analysis of a PI-resistant tomato cultivar Micro-Tom (MT) shows a relationship between PI and accumulation of toxic leaf nitrite. Nitrite is formed by nitrate reductase (NR) and converted to ammonium by nitrite reductase (NiR). We hypothesized that a decrease in the NiR:NR activity ratio predisposes plants to PI. MT maintained its normal circadian rhythms for NR and NiR activity even in 24-h light, whereas these enzyme activities became arrhythmic in a PI-susceptible line, and NiR:NR activity ratio decreased. To determine if MT can become PI-susceptible, the Micallef lab generated several transgenic Micro-Tom lines that contain a DEX-inducible antisense construct for NiR. NiR transcript levels and enzyme activity were reduced in the transgenic lines, and transgenic plants grown under an extended photoperiod exhibited PI. en_US
dc.language.iso en en_US
dc.subject Photoperiodic injury en_US
dc.subject Circadian clock en_US
dc.subject Tomato en_US
dc.subject Solanum lycopersicum en_US
dc.subject Micro-Tom en_US
dc.subject BasketVee en_US
dc.subject Nitrite reductase en_US
dc.subject Nitrate reductase en_US
dc.subject Nitrite en_US
dc.subject Nitrite toxicity en_US
dc.subject Continuous light en_US
dc.subject Alternating photoperiod en_US
dc.subject PI-resistant en_US
dc.subject PI-susceptible en_US
dc.subject Photoperiod en_US
dc.subject NiR:NR ratio en_US
dc.title Establishing the Relationship Between Nitrogen Assimilation and Photoperiodic Injury in Tomato (Solanum lycopersicum L.) en_US
dc.type Thesis en_US
dc.degree.programme Plant Agriculture en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Plant Agriculture en_US


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