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Anti-inflammatory Activity of Heat-killed Probiotic, Lactobacillus pentosus S-PT84 in a C57BL/6J Mouse Model with LPS-induced Low-grade Chronic Inflammation

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Title: Anti-inflammatory Activity of Heat-killed Probiotic, Lactobacillus pentosus S-PT84 in a C57BL/6J Mouse Model with LPS-induced Low-grade Chronic Inflammation
Author: Zeng, Yuhan
Department: Department of Food Science
Program: Food Science
Advisor: Mine, Yoshinori
Abstract: This study is aimed to identify the anti-inflammatory activity and mechanism of the heat-killed probiotic, Lactobacillus pentosus S-PT84, in a mouse model with lipopolysaccharide (LPS)-induced systemic low-grade chronic inflammation. The persistent increase of intestinal permeability contributes to the development of intestinal inflammation and systemic inflammation. Systemic low-grade chronic inflammation is tightly related to insulin resistance and metabolic diseases. Pre-treatment of Lactobacillus pentosus S-PT84 decreased the production of pro-inflammatory mediators, TNF-α and MCP-1, and stimulated the production of anti-inflammatory mediator, adiponectin in plasma by increasing the protein expression of peroxisome proliferator-activated receptor gamma (PPAR-γ) in the white adipose tissues. Lactobacillus pentosus S-PT84 maintained the structural integrity of intestines and homeostasis of intestinal permeability via modulating the expression of tight junction proteins. The intact integrity of mucosal barrier reduced the entrance and content of inflammatory stimulants in the circulating system. Moreover, Lactobacillus pentosus S-PT84 exerted the protective effects on preventing the insulin resistance by increasing the insulin sensitivity, glucose tolerance, and protein expression of insulin receptor subset-1 (IRS-1). This study provides an evidence that Lactobacillus pentosus S-PT84 possesses anti-inflammatory properties and exerts beneficial functions to prevent systemic low-grade chronic inflammation and reduces the risk of developing insulin resistance and associated metabolic diseases.
URI: http://hdl.handle.net/10214/11581
Date: 2017-09-13


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Attribution 2.5 Canada Except where otherwise noted, this item's license is described as Attribution 2.5 Canada