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Antioxidant and Antiproliferative Activity of Flavonoids from Ontario Grown Onions by Pressurized Low Polarity Water Technology

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Title: Antioxidant and Antiproliferative Activity of Flavonoids from Ontario Grown Onions by Pressurized Low Polarity Water Technology
Author: Manohar, Cynthya
Department: School of Engineering
Program: Engineering
Advisor: Neethirajan, Suresh
Abstract: Naturally occurring plant flavonoids are in great demand in both nutra-pharmaceutical and biomedical industries. Flavonoids are a group of phytochemicals that have shown various potential health benefits and are found abundantly in onion. The present study is to screen Ontario grown onion varieties, namely Stanley, Ruby Ring, Safrane, Fortress and Lasalle, and test their antioxidant and antiproliferative capacities. Pressurized low polarity water technology is an eco-friendly technique used for extracting polyphenolic compounds from these onion varieties, followed by quantification and analysis using High performance liquid chromatography. The antioxidant activities in the test samples were measured using various antioxidant assays. Ruby Ring - a red onion variety - exhibited high phenolic and antioxidant activities when compared to the other yellow onion varieties tested. The antiproliferative activity of onion extracts against Caco-2 cancer cells were assessed through cytotoxic, apoptosis and anti-migratory assays. Stanley - a yellow onion variety - showed the highest anticancer activity against Caco-2 cells. Since there was an increase in both radical scavenging activity and total phenolic compounds by Ruby Ring, this red onion variety may be chosen as a preferred variety for developing functional food products.
URI: http://hdl.handle.net/10214/10241
Date: 2017-02-09


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